Pdf ebook: Flow Cytometry and Cell Sorting

Pdf download ebook Flow Cytometry and Cell Sorting

Summary: The practical aspects of flow cytometry and sorting are emphasized in this book which introduces the beginner to the technology and provides tips and trick

  • Author : N.a
  • Publisher: Springer Science & Business Media
  • ISBN: 3662027852
  • Genre: Science
  • Number of Pages: 223
  • Language: English
  • Views: 1013
  • Downloads: 1013
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Description: The practical aspects of flow cytometry and sorting are emphasized in this book which introduces the beginner to the technology and provides tips and tricks for the advanced user. The clear structure makes it easy to address specific problems fast. The chapters cover the modern applications of these procedures, with emphasis on immunofluorescence (antibody-fluorochrome conjugation, staining principles and data evaluation); the isolation of specific chromosomes, cells and fragile, large particles by magnetic and fluorescence-activated sorting; cellular biochemistry; and the dynamics of proliferation. The methods have been field-tested in recent EMBO courses on flow cytometry.


Pdf ebook: Flow Cytometry and Cell Sorting

Pdf download ebook Flow Cytometry and Cell Sorting

Summary: The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this

  • Author : Andreas Radbruch
  • Publisher: Springer Science & Business Media
  • ISBN: 3662041294
  • Genre: Science
  • Number of Pages: 355
  • Language: English
  • Views: 1451
  • Downloads: 1451
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Description: The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS


Pdf ebook: In Living Color

Pdf download ebook In Living Color

Summary: A contribution towards making this increasingly valuable technology accessible to researchers, including the students, post-doctoral scholars, and technici

  • Author : Rochelle A. Diamond,Susan DeMaggio
  • Publisher: Springer Science & Business Media
  • ISBN: 3642570496
  • Genre: Science
  • Number of Pages: 800
  • Language: English
  • Views: 400
  • Downloads: 400
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Description: A contribution towards making this increasingly valuable technology accessible to researchers, including the students, post-doctoral scholars, and technicians gathering the knowledge inherent in this integration between analysis and physical isolation/purification methodologies. A step-by-step approach to the methodology for measuring various attributes demonstrated in the particular cells of interest is provided, as is a myriad of resources to fuel the curiosity and answer questions of both new and adept users. This book stems from the editors'experiences managing flow cytometry/cell sorting core facilities for the emerging researchers, in particular in developmental, cellular, and molecular biology.


Pdf ebook: Flow Cytometry

Pdf download ebook Flow Cytometry

Summary: Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a cle

  • Author : Marion G. Macey
  • Publisher: Springer Science & Business Media
  • ISBN: 1597454516
  • Genre: Medical
  • Number of Pages: 294
  • Language: English
  • Views: 1207
  • Downloads: 1207
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Description: Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.


Pdf ebook: Cell Separation

Pdf download ebook Cell Separation

Summary: With contributions by numerous experts

  • Author : Ashok Kumar,Igor Yu Galaev,Bo Mattiasson
  • Publisher: Springer
  • ISBN: 3540752633
  • Genre: Science
  • Number of Pages: 203
  • Language: English
  • Views: 1903
  • Downloads: 1903
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Description: With contributions by numerous experts


Pdf ebook: Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

Pdf download ebook Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

Summary: A much-needed primer on the use of laser flow cytometry for stemcell analysis Laser flow cytometry is a powerful tool for rapid analysis ofcells for marker

  • Author : Awtar Krishan,H. Krishnamurthy,Satish Totey
  • Publisher: John Wiley & Sons
  • ISBN: 1118148061
  • Genre: Science
  • Number of Pages: 410
  • Language: English
  • Views: 1020
  • Downloads: 1020
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Description: A much-needed primer on the use of laser flow cytometry for stemcell analysis Laser flow cytometry is a powerful tool for rapid analysis ofcells for marker expression, cell cycle position, proliferation,and apoptosis. However, no resources specifically address the useof this methodology for the study of stem cells; this is especiallyimportant as stem cell analysis involves specialized methods andstaining procedures based on specific characteristics such asmarker expression, cell size, drug transport, and efflux of thestem cells. Now, this book reviews these procedures, discusses the sciencebehind them, and provides real-world examples to illustrate theusefulness of the methods. It brings together world-class expertsin pathology, biophysics, immunology, and stem cell research, whodraw upon their extensive experience with the methods and showexamples of good data to help guide researchers in the rightdirection. Chapter coverage includes: Stem cell analysis and sorting using side population Flow cytometry in the study of proliferation and apoptosis Stem cell biology and application Identification and isolation of very small embryonic-like stemcells from murine and human specimens Hematopoietic stem cells—issues in enumeration Human embryonic stem cells: long-term culture andcardiovascular differentiation Limbal stem cells and corneal regeneration Flow cytometric sorting of spermatogonial stem cells Breast cancer stem cells Stem cell marker expression in cells from body cavityfluids This book is an essential resource for all graduate students,practitioners in developing countries, libraries and bookrepositories of universities and research institutions, andindividual researchers. It is also of interest to laboratoriesengaged in stem cell research and use of stem cells for tissueregeneration, and to any organization dealing in stem cell andtissue regeneration research.


Pdf ebook: Practical Flow Cytometry

Pdf download ebook Practical Flow Cytometry

Summary: From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinic

  • Author : Howard M. Shapiro
  • Publisher: John Wiley & Sons
  • ISBN: 0471434035
  • Genre: Science
  • Number of Pages: 736
  • Language: English
  • Views: 1451
  • Downloads: 1451
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Description: From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.


Pdf ebook: Flow Cytometry

Pdf download ebook Flow Cytometry

Summary: Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science,

  • Author : Alice Longobardi Givan
  • Publisher: John Wiley & Sons
  • ISBN: 1118688392
  • Genre: Science
  • Number of Pages: 296
  • Language: English
  • Views: 511
  • Downloads: 511
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Description: Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.


Pdf ebook: Flow Cytometry with Plant Cells

Pdf download ebook Flow Cytometry with Plant Cells

Summary: Targeted at beginners as well as experienced users, this handy reference explains the benefits and uses of flow cytometery in the study of plants and their

  • Author : Jaroslav Dolezel,Johann Greilhuber,Jan Suda
  • Publisher: John Wiley & Sons
  • ISBN: 3527610936
  • Genre: Science
  • Number of Pages: 479
  • Language: English
  • Views: 584
  • Downloads: 584
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Description: Targeted at beginners as well as experienced users, this handy reference explains the benefits and uses of flow cytometery in the study of plants and their genomes. Following a brief introduction that highlights general considerations when analyzing plant cells by flow cytometric methods, the book goes on to discuss examples of application in plant genetics, genomic analysis, cell cycle analysis, marine organism analysis and breeding studies. With its list of general reading and a glossary of terms, this first reference on FCM in plants fills a real gap by providing first-hand practical hints for the growing community of plant geneticists.


Pdf ebook: Flow Cytometry, Part B

Pdf download ebook Flow Cytometry, Part B

Summary: From the Reviews of the First Edition: "This is a good reference manual for multi-user facility faced with a wide variety of biological applications." - CY

  • Author : N.a
  • Publisher: Elsevier
  • ISBN: 0080859402
  • Genre: Science
  • Number of Pages: 697
  • Language: English
  • Views: 925
  • Downloads: 925
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Description: From the Reviews of the First Edition: "This is a good reference manual for multi-user facility faced with a wide variety of biological applications." - CYTOMETRY "Flow Cytometry includes an impressive array of methods applicable to chromosome analysis, plant biology, marine biology, fluorescence, insitu hybridization, and others. It succeeds in providing the reader with good insight into the power of the technology throughout biology." - KENNETH A. AULT, MAINE CYTOMETRY RESEARCH INSTITUTE, MAINE MEDICAL CENTER, IN CANCER CELLS Flow Cytometry, Second Edition provides a complete and comprehensive two-volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who use flow Cytometry to separate and analyze cells. * * Comprehensive methodological coverage in unique style * In depth treatment of procedures * Description of each procedure's: * Theoretical foundations * Critical aspects * Possible pitfalls * Written by authors with extensive experience who: * Developed or modified the technique * Describe their experience with different instruments and applications to different cell systems * Are the Who's Who in Flow Cytometry * 10 methods cover assessment of apoptosis and other modes of cell death * Practical, handbook-style presentation works in lab or classroom * Printed on acid-free paper * Color plates


Pdf ebook: The Machinery of Life

Pdf download ebook The Machinery of Life

Summary: A journey into the sub-microscopic world of molecular machines. Readers are first introduced to the types of molecules built by cells: proteins, nucleic ac

  • Author : David S. Goodsell
  • Publisher: Springer Science & Business Media
  • ISBN: 1475722672
  • Genre: Science
  • Number of Pages: 140
  • Language: English
  • Views: 1611
  • Downloads: 1611
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Description: A journey into the sub-microscopic world of molecular machines. Readers are first introduced to the types of molecules built by cells: proteins, nucleic acids, lipids, and polysaccharides. Then, in a series of distinctive illustrations, the reader is guided through the interior world of cells, exploring the ways in which molecules work in concert to perform the processes of living. Finally, the author shows us how vitamins, viruses, poisons, and drugs each have their effects on the molecules in our bodies. David Goodsell, author and illustrator, has prepared a fascinating introduction to biochemistry for the non-specialist. His book combines a lucid text with an abundance of drawings and computer graphics that present the world of cells and their components in a truly unique way.


Pdf ebook: Single Cell Sequencing and Systems Immunology

Pdf download ebook Single Cell Sequencing and Systems Immunology

Summary: The volume focuses on the genomics, proteomics, metabolomics, and bioinformatics of a single cell, especially lymphocytes and on understanding the molecula

  • Author : Xiangdong Wang
  • Publisher: Springer
  • ISBN: 9401797536
  • Genre: Medical
  • Number of Pages: 177
  • Language: English
  • Views: 1945
  • Downloads: 1945
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Description: The volume focuses on the genomics, proteomics, metabolomics, and bioinformatics of a single cell, especially lymphocytes and on understanding the molecular mechanisms of systems immunology. Based on the author’s personal experience, it provides revealing insights into the potential applications, significance, workflow, comparison, future perspectives and challenges of single-cell sequencing for identifying and developing disease-specific biomarkers in order to understand the biological function, activation and dysfunction of single cells and lymphocytes and to explore their functional roles and responses to therapies. It also provides detailed information on individual subgroups of lymphocytes, including cell characters, function, surface markers, receptor function, intracellular signals and pathways, production of inflammatory mediators, nuclear receptors and factors, omics, sequencing, disease-specific biomarkers, bioinformatics, networks and dynamic networks, their role in disease and future prospects. Dr. Xiangdong Wang is a Professor of Medicine, Director of Shanghai Institute of Clinical Bioinformatics, Director of Fudan University Center for Clinical Bioinformatics, Director of the Biomedical Research Center of Zhongshan Hospital, Deputy Director of Shanghai Respiratory Research Institute, Shanghai, China.


Pdf ebook: Advanced Flow Cytometry: Applications in Biological Research

Pdf download ebook Advanced Flow Cytometry: Applications in Biological Research

Summary: Flow cytometry has rapidly evolved into a technique for rapid analysis of DNA content, cellular marker expression and electronic sorting of cells of intere

  • Author : R.C. Sobti,A. Krishan
  • Publisher: Springer Science & Business Media
  • ISBN: 9401706239
  • Genre: Medical
  • Number of Pages: 114
  • Language: English
  • Views: 1933
  • Downloads: 1933
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Description: Flow cytometry has rapidly evolved into a technique for rapid analysis of DNA content, cellular marker expression and electronic sorting of cells of interest for further investigations. Flow cytometers are being extensively used for monitoring of cellular DNA content, phenotype expression, drug transport, calcium flux, proliferation and apoptosis. Phenotypic analysis of marker expression in leukemic cells has become an important tool for diagnostic and therapeutic monitoring of patients. Recent studies have explored the use of flow cytometry for monitoring hormone receptor expression in human solid tumors and for studies in human genomics. Contributions in the current volume are based on presentations made at the First Indo-US workshop on Flow Cytometry in which experts from USA, UK and India discussed applications of flow cytometry in biological and medical research. This book will be of interest to post graduates and researchers in the fields of pathology, cytology, cell biology and molecular biology.


Pdf ebook: Cell Separation

Pdf download ebook Cell Separation

Summary: With contributions by numerous experts

  • Author : Ashok Kumar,Igor Yu Galaev,Bo Mattiasson
  • Publisher: Springer
  • ISBN: 3540752633
  • Genre: Science
  • Number of Pages: 203
  • Language: English
  • Views: 1268
  • Downloads: 1268
  • GET BOOK

Description: With contributions by numerous experts


Pdf ebook: Essential Cytometry Methods

Pdf download ebook Essential Cytometry Methods

Summary: Cytometry is characterization and measurement of cells and cellular constituents, most often used to immunophenotype cells - that is, to distinguish health

  • Author : Zbigniew Darzynkiewicz,J. Paul Robinson,Mario Roederer
  • Publisher: Academic Press
  • ISBN: 9780123750464
  • Genre: Science
  • Number of Pages: 920
  • Language: English
  • Views: 361
  • Downloads: 361
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Description: Cytometry is characterization and measurement of cells and cellular constituents, most often used to immunophenotype cells - that is, to distinguish healthy cells from diseased cells. Flow Cytometry specifically is quite sensitive, allowing researchers to detect rare cell types and residual levels of disease, and as such has been the method of choice for important studies such as monitoring the blood of AIDS patients. For this reason, there is a great need for a practical, comprehensive manual that will be useful across a broad range of laboratories. This volume, as part of the Reliable Lab Solution Series, delivers such a tool, offering busy researchers across many disciplines a handy resource of all the best methods and protocols for Cytometry to use at the bench. * Highlights top downloaded and cited chapters, authored by pioneers in the field and enhanced with their tips, and pitfalls to avoid. * Loaded with detailed protocols developed and used by leaders in the field. *Refines, organizes and updates popular methods from one of our top selling series, Methods in Cell Biology


Pdf ebook: Microfluidics and Microfabrication

Pdf download ebook Microfluidics and Microfabrication

Summary: Microfluidics and Microfabrication discusses the interconnect between microfluidics, microfabrication and the life sciences. Specifically, this includes fu

  • Author : Suman Chakraborty
  • Publisher: Springer Science & Business Media
  • ISBN: 1441915435
  • Genre: Technology & Engineering
  • Number of Pages: 357
  • Language: English
  • Views: 834
  • Downloads: 834
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Description: Microfluidics and Microfabrication discusses the interconnect between microfluidics, microfabrication and the life sciences. Specifically, this includes fundamental aspects of fluid mechanics in micro-scale and nano-scale confinements and microfabrication. Material is also presented discussing micro-textured engineered surfaces, high-performance AFM probe-based, micro-grooving processes, fabrication with metals and polymers in bio-micromanipulation and microfluidic applications. Editor Suman Chakraborty brings together leading minds in both fields who also: Cover the fundamentals of microfluidics in a manner accessible to multi-disciplinary researchers, with a balance of mathematical details and physical principles Discuss the explicit interconnection between microfluiodics and microfabrication from an application perspective Detail the amalgamation of microfluidics with logic circuits and applications in micro-electronics Microfluidics and Microfabrication is an ideal book for researchers, engineers and senior-level graduate students interested in learning more about the two fields.


Pdf ebook: Cell Separation Methods and Applications

Pdf download ebook Cell Separation Methods and Applications

Summary: "Offers complete coverage and assessment of cell separation technologies for analytical and preparative isolations of biological cell populations-demonstra

  • Author : Diether Recktenwald
  • Publisher: CRC Press
  • ISBN: 1482273640
  • Genre: Medical
  • Number of Pages: 352
  • Language: English
  • Views: 598
  • Downloads: 598
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Description: "Offers complete coverage and assessment of cell separation technologies for analytical and preparative isolations of biological cell populations-demonstrating how to select and devise optimal sorting strategies for applications in biochemistry, immunology, cell and molecular biology, and clinical research. "


Pdf ebook: Single-Cell Omics

Pdf download ebook Single-Cell Omics

Summary: Single-Cell Omics: Volume 1: Technological Advances and Applications provides the latest technological developments and applications of single-cell technol

  • Author : Debmalya Barh,Vasco Azevedo
  • Publisher: Academic Press
  • ISBN: 0128149205
  • Genre: Business & Economics
  • Number of Pages: 490
  • Language: English
  • Views: 706
  • Downloads: 706
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Description: Single-Cell Omics: Volume 1: Technological Advances and Applications provides the latest technological developments and applications of single-cell technologies in the field of biomedicine. In the current era of precision medicine, the single-cell omics technology is highly promising due to its potential in diagnosis, prognosis and therapeutics. Sections in the book cover single-cell omics research and applications, diverse technologies applied in the topic, such as pangenomics, metabolomics, and multi-omics of single cells, data analysis, and several applications of single-cell omics within the biomedical field, for example in cancer, metabolic and neuro diseases, immunology, pharmacogenomics, personalized medicine and reproductive health. This book is a valuable source for bioinformaticians, molecular diagnostic researchers, clinicians and members of the biomedical field who are interested in understanding more about single-cell omics and its potential for research and diagnosis. Covers not only the technological aspects, but also the diverse applications of single cell omics in the biomedical field Summarizes the latest progress in single cell omics and discusses potential future developments for research and diagnosis Written by experts across the world, bringing different points-of-view and case studies to give a comprehensive overview on the topic


Pdf ebook: Microfluidic Devices for Biomedical Applications

Pdf download ebook Microfluidic Devices for Biomedical Applications

Summary: Microfluidics or lab-on-a-chip (LOC) is an important technology suitable for numerous applications from drug delivery to tissue engineering. Microfluidic d

  • Author : Xiujun James Li,Yu Zhou
  • Publisher: Elsevier
  • ISBN: 0857097040
  • Genre: Technology & Engineering
  • Number of Pages: 676
  • Language: English
  • Views: 1656
  • Downloads: 1656
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Description: Microfluidics or lab-on-a-chip (LOC) is an important technology suitable for numerous applications from drug delivery to tissue engineering. Microfluidic devices for biomedical applications discusses the fundamentals of microfluidics and explores in detail a wide range of medical applications. The first part of the book reviews the fundamentals of microfluidic technologies for biomedical applications with chapters focussing on the materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies. Chapters in part two examine applications in drug discovery and controlled-delivery including micro needles. Part three considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering. The final part of the book covers the applications of microfluidic devices in diagnostic sensing, including genetic analysis, low-cost bioassays, viral detection, and radio chemical synthesis. Microfluidic devices for biomedical applications is an essential reference for medical device manufacturers, scientists and researchers concerned with microfluidics in the field of biomedical applications and life-science industries. Discusses the fundamentals of microfluidics or lab-on-a-chip (LOC) and explores in detail a wide range of medical applications Considers materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies Considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering


Pdf ebook: Flow Cytometry in Drug Discovery and Development

Pdf download ebook Flow Cytometry in Drug Discovery and Development

Summary: This book covers the unique application of flow cytometry in drug discovery and development. The first section includes two introductory chapters, one on f

  • Author : Virginia Litwin,Philip Marder
  • Publisher: John Wiley & Sons
  • ISBN: 0470922788
  • Genre: Medical
  • Number of Pages: 360
  • Language: English
  • Views: 881
  • Downloads: 881
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Description: This book covers the unique application of flow cytometry in drug discovery and development. The first section includes two introductory chapters, one on flow cytometry and one on biomarkers, as well as a chapter on recent advances in flow cytometry. The second section focuses on the unique challenges and added benefits associated with the use of flow cytometry in the drug development process. The third section contains a single chapter presenting an in depth discussion of validation considerations and regulatory compliance issues associated with drug development.